| 1. | The pichia pastoris recombinant strain was induced by methanol addition to the culture medium
Sds - page分析表明，在63kd处有明显的蛋白质特征带。 |
| 2. | Expression of cyt1aa from bacillus thuringiensis in bacillus thuringiensis var . tenebrionis strain and the insecticidal activity of the recombinant strain
基因在拟步甲亚种菌株中的表达及重组菌株杀虫活性研究 |
| 3. | The gene recombinant strain no . 42 ca n ' t generate ampramycin , which indicated that the cloned gene is involved in apramycin biosynthesis in s . tenebrarius
通过接合转移的方法将质粒pzxb014导入黑暗链霉菌h6中，筛选基因发生重组的菌株。 |
| 4. | Finally , the growth conditions , growth curves , fermentation conditions of recombinant strains and the biological properties of purified recombinant protein were investigated
最后对重组菌的生长条件、生长曲线、发酵条件和表达产物的体外生物学活性进行了研究。 |
| 5. | Some important factors of high density fermentation of genetic engineering microorganisms including the construct of recombinant strains , culture conditions , growth inhibitor and the process control were described
摘要阐述了基因工程菌高密度发酵工艺的几个主要影响因素，包括重组菌构建、培养条件、生长抑制因数以及它们的控制技术。 |
| 6. | Object : the culture medium and culture conditions of psb will be optimized and gene ubia will be cloned for the construction of recombinant strain producing and foundation of the fermentative technology in large scale
目的：优化psb产coq _ ( 10 )培养基及培养条件和克隆ubia基因，为获取高产coq _ ( 10 )基因工程菌及确定规模化发酵工艺奠定良好的基础。 |
| 7. | The three recombinant strains were induced to express with iptg . the tannase activity was detected by ultravillet spectroscopy and the target protein was found in sds - page with the correct size of 63kd . the gene was also insered in the plasmid ppic9k of pichia pastoris
将单宁酶基因tan重组到trc启动子控制下的表达载体pse380中，并分别转化入大肠杆菌dh5 ， top10 ， bl21 ( de3 )三种重组表达菌株中。 |
| 8. | One highly productive and genetically stable recombinant strain named e - 22 , which produced phytase with 143958 . 3u / ml under the condition of flask cultivation , was selected through further screening . the phytase activity of e - 22 was 341 . 13 times as high as that of the original strain ( 422u / ml )
经摇瓶复筛后得到l株产酶活性为143958 . 3uzml发酵液，并且具有良好遗传稳定性的高产工程菌株( e22 ) ，其产酶活性是出发菌株酶活性( 422u / ml )的341 |
| 9. | The genes encoding pyruvate decarboxylase ( pdc ) and alcohol dehydrogenase ii ( adh ii ) were amplified from total dna of zymomonas mobilis by pcr . the genes of adhb andpdc were inserted into expression vector pse380 and then transformed into e . coli dh5a . the recombinant strains were induced to express adh ii and pdc with iptg
本论文以zymomonasmobilisdna为模板， pcr扩增zymomonasmobilis中的乙醇脱氢酶基因( adhb )和丙酮酸脱羧酶基因( pdc ) ，分别构建表达质粒pse - adhb和pse - pdc并在大肠杆菌dh5中表达。 |
| 10. | It was confirmed that the key enzyme of coq10 synthesis is p - hydroxybenzoate polyprenyltransferase , encoded by gene ubia in e . coli and lacks specificity to the aggregation length of its substrate ( polyisoprenyl pyrophosphate ) . if gene ubia can be introduced into psb and is high expression in psb , a recombinant strain producing coq10 could be obtained . consequently , the productive cost will decrease sharply
业已证实coq _ ( 10 )生物合成的关键酶为对羟基苯甲酸聚异戊二烯焦磷酸转移酶(在大肠杆菌中该酶由ubia基因编码) ，该类酶对底物聚异戊二烯焦磷酸( ppp )的聚合长度并无特殊要求。 |
